Comparison of hemodynamic change following spinal anesthesia in preeclamptic and non-preeclamptic parturients undergoing cesarean section at University of Gondar comprehensive specialized hospital, Northwest Ethiopia.

Background Spinal anesthesia-induced maternal hypotension is the most frequent complication associated with maternal morbidity and mortality during cesarean section. The aim of this study was to compare the incidence and magnitude of hemodynamic changes in preeclamptic and non-preeclamptic parturients undergone cesarean section under spinal anesthesia. Method A prospective cohort study was conducted from February to May 2019 in University of Gondar comprehensive specialized hospital. A total of 122 ASA II and ASA III parturients were recruited consecutively and assigned to two groups (81non-preeclamptics, and 41 preeclamptics). The data analysis was done by SPSS version 22 statistical software. The data were tested for normality with Shapiro Wilk U-test and normally distributed data were compared by using the independent student’s t-test. Whereas non-normally distributed data were compared using the Mann-Whitney U- test. Fisher’s exact test was used for intergroup comparison of proportion. All P values <0.05 were considered statistically significant.


Background
Worldwide preeclampsia/eclampsia is the third leading cause of maternal morbidity and mortality [1]. This is especially the commonest cause of fetomaternal complications in developing countries; around 40-60% of maternal deaths in these countries are caused by preeclampsia alone; in Ethiopia,19% of maternal mortality is caused by hypertensive disorder of pregnancy [2]. The definitive management of preeclampsia is delivery where cesarean section is commonly promoted [3]. A study done in Ethiopia showed that 6.1% of indications for CS were preeclampsia [4]. There is a paradigm shift in the practice of obstetrics anesthesia from general to spinal anesthesia for cesarean section, but the rate of preventable spinal anesthesia-related deaths is still high [5][6][7]. Hypotension after spinal anesthesia was the most frequent complication associated with maternal morbidity and mortality during cesarean section [8]. Preeclamptic patients have been considered to be at higher risk of profound hypotension when they undergo cesarean section under spinal anesthesia [9].
A study had shown that fluid loading and vasopressor prophylaxis were effective in reducing the incidence of spinal anesthesia-induced hypotension in healthy parturients [11]. But these preemptive measures could put the preeclamptic patients at increased risk of hypertension and pulmonary edema [9]. Due to inconsistent definition, the reported incidence of spinal anesthesia-induced maternal hypotension varies between 7% and 89.2% [10,16]. Therefore, anesthetic management for preeclamptic parturients who undergo cesarean section is challenging for anesthetists [12]. To alleviate maternal deaths arise from risks attributable to pregnancy and childbirth, the provision of safe anesthesia is necessitating [3,19,24].
During obstetric anesthesia, preservation of hemodynamic stability is a big concern for anesthetists [12], especially for preeclamptic parturients who planned to undergo cesarean section [10,12]. However, spinal anesthesia-induced maternal hypotension is still the most frequent complication [20,21]. Anesthetists denied spinal anesthesia for preeclamptic parturients, due to the fear of profound hypotension and its management crisis (exaggerated response to vasopressor treatment and pulmonary edema following fluid challenges) [9,[12][13][14][15]. Furthermore, the incidence of spinal anesthesia-induced maternal hypotension showed inconsistency across different studies, which makes it almost difficult to set standard targets and develop a local management protocol [20,21,33,34].

Methods And Materials
A prospective observational cohort study was conducted to determine the incidence of hypotension and the magnitude of hemodynamics change following spinal anesthesia in preeclamptic and non-preeclamptic parturients undergone cesarean section in University of Gondar comprehensive specialized hospital; Northwest Ethiopia from January to June 2019. ASA II and ASA III parturients were involved in the study. Parturients with cardiac disease, twin pregnancy, total spinal, chronic hypertension, gestational hypertension, superimposed hypertension, renal disease, diabetes mellitus, coagulopathy (platelet count < 80 × 10 9 /L), active labor, eclampsia, abruptio placentae, placenta praevia, any adjuvant added with local anesthetics were excluded. Variables like age, height, BMI, ASA status, gestational age, and amount of fluid preloaded, amount of fluid consumed intraoperatively, the weight of the neonate, upper sensory level of the spinal block at the time of skin incision, position during and after the spinal procedure were studied.
Preeclampsia with severity feature/severe preeclampsia: Defined as a systolic arterial blood pressure of 160 mmHg or higher, or a diastolic blood pressure of 110 mmHg or higher, associated with proteinuria > 5 g in 24 hours [10,35]. Change in heart rate: When there was a 20% decrease or increase in heart rate from baseline [6,10,25,26,36]. The magnitude of hemodynamics change/severity of hypotension: The percentage falls of blood pressure (SBP, DBP, and MAP) between two measurements and it was calculated as: Percentage fall = [10,25,37].
All consecutive parturients who fulfilled the inclusion criteria and gave birth by cesarean section under spinal anesthesia were included in the study until the intended sample size has been achieved. The sample size was determined based on the latest study done in Macedonia the incidence of hypotension in preeclamptic and non-preeclamptic parturients was 25% and 53% respectively [25]and calculated by using the Fleiss correction factor method with a power of 80% at a 5% significance level and the sample size was 122.
Hereafter, 41 participants were enrolled in the preeclamptic group and 81 participants were enrolled in non-preeclamptic groups with a proportion of 1:2 ratios respectively. All parturients who satisfy the inclusion criteria were included in the study period. There was homogeneity of variance, as assessed by Levene's test for equality of variances.
In the operation theater, baseline hemodynamic variables (SBP, DBP, MAP, and HR) were recorded. Baseline BP was taken as the mean of the two readings measured one minute apart and five minutes after the parturient arrived in the operation theatre and before doing any invasive procedures. After spinal anesthesia SBP, DBP, MAP, and HR were recorded every 2 minutes for 30 minutes and every 5 minutes thereafter until the end of surgery. Patients were monitored with non-invasive automated blood pressure cuffs, ECG, and pulse oximetry. The data collectors have assessed the upper level of sensory block bilaterally by pinprick at the time of skin incision and it was documented. The total intraoperative fluid consumption, total estimated blood loss, the weight of the newborn were documented as well. The data collection technique was a combination of chart review, observation, and interview using a pre-tested questioner that was developed in English language.
After completion of the data collection, the data was entered into Epidata version 4.2.
Software and exported to SPSS version 22 statistical software for further analysis. The data were tested for normality with Shapiro Wilk U-test and normally distributed data were compared by using the independent student's t-test and expressed as mean ± SD.
Whereas non-normally distributed data were compared using the Mann-Whitney U-test and expressed as medians(IQR). Fisher's exact test was used for intergroup comparisons of proportion. All P values < 0.05 were considered significant. The research was taken ethical clearance from University of Gondar College of medicine and health science ethical review board and written informed consent was taken from each study participants.

Results
A total of 122 parturients were enrolled (81 non-preeclamptic and 41 preeclamptic parturients) in this study. There were no statistically significant differences in socio-demographic and anesthetic characteristics of parturients such as; age, weight, height, the volume of 0.5% plain bupivacaine, and speed of spinal administration between groups (Tables 1 and 3). The majority of preeclamptic parturients were ASA II and the remains were ASA III, while, All parturients in the non-preeclamptic group were ASA II, and this difference was statistically significant between groups; p < 0.001 (Table 1). The mean gestational age at the time of cesarean section was significantly lower in the preeclamptic group: 38.56 ± 1.63 weeks in non-preeclamptic versus 37.44 ± 1.25 weeks in preeclamptics; p = 0.001 (Table 1). However, there was no significant difference in the mean weight of the newborn between groups; p = 0.37([ Table 1).  The median upper sensory level at the time of skin incision was higher in the preeclamptic parturients compared to those with non-preeclamptics and this difference was statistically significant (T5 vs. T6; p = 0.032) ( Table 3). The baseline SBP, DBP, MAP, and heart rate were higher in parturients with preeclampsia than the corresponding values among the non-preeclamptic parturients ( there was a statistically significant difference in intraoperative intravenous fluid consumption between groups, which was higher in non-preeclamptics compared to preeclamptic parturients (1723.46 ml ± 352.41vs1463.41 ± 417.59; p = 0.001) ( Table 4). The mean duration of surgery was comparable between the two groups ( Table 4).  In the preeclamptic parturients, mean SBP and DBP were higher than the corresponding values among non-preeclamptic parturients following spinal anesthesia at each point of time (Fig. 3, 4) and the same fashion was happening to MAP, which was at a higher level in preeclamptic parturients than non-preeclamptic parturients (Fig. 2). The incidence of hypotension in non-preeclamptic parturients (55.6%) was higher than that of preeclamptic parturients (34.1%) (
Nikooseresht M et al. found that SBP, DBP, and MAP measured at the baseline were higher for the patients with preeclampsia, and the lowest mean SBP, DBP, and MAP measured among the preeclamptic patients were higher than the corresponding values among the healthy parturients [10].
This finding was in line with our study result. In this study, the incidence of hypotension after spinal anesthesia in preeclamptic parturients (34.1%) was less than that of non-preeclamptic parturients (55.6%) (p =0.035). This discrepancy in the incidence of hypotension related to preeclampsia related factors. Despite the sympathetic block due to spinal anesthesia, because of exaggerated vasoconstriction, preeclamptic parturients can still maintain their vascular tone that caused only a limited decrease in blood pressure.  [37]. The possible explanation for this discrepancy might be the employment of invasive blood pressure monitoring in their study, in contrast to our study. Similar to our study Aya AG et al. found that severely preeclamptic patients had a less frequent incidence of clinically significant hypotension compared to healthy parturients(16.6% versus 53.3%;P=0.006 [39]. The incidence of hypotension among preeclamptic parturients in our study was higher than Aya AG et al. result. The likely reason may be the use of different criteria for defining hypotension (20% versus 30% decline to baseline MAP) and the use of the small volume of preload in our study participants compared to Aya AG et al. (565.38ml ± 318.4 vs1653 ml±331).
In contradict to our result, Mendes et al. found that there was no statistically significant difference regarding the occurrence of hypotension after spinal anesthesia between severely preeclamptic and healthy parturients. But the incidence rate of hypotension was high in both groups(84%and70%,p=0.45) [26]. This difference may be due to the intraoperative administration of intravenous hydralazine in preeclamptic parturients in their study.
Sivevski A et al. found that the percentage of fall of BP from baseline were significantly greater in the healthy parturients compared to those with preeclampsia (25.8% ± 10.1 vs. 18.8% ± 17.0 for SBP, 28.5% ± 8.8 vs. 22.5% ± 10.4 for DBP, and 31.2% ± 14.2 vs. 18.2% ± 12.6% for MAP, p < 0.05 [25]. Likewise, another study conducted by Saha D et al. found that the percentage of fall of DBP and MAP calculated from the baseline was also less in the preeclamptic group (34.5% and 33% in normotensive as opposed to 30.3% and 32.3% in preeclamptics, respectively) [1]. The result of our study was in accordance with the above findings.
Unlike our study, Mendes et al. found that there was no significant difference in the lowest mean drop of SBP and DBP after spinal anesthesia between preeclamptic and healthy parturients [26]. This difference may be due to standardized fluid management and administration of potent direct vasodilator during surgery (intravenous hydralazine) in preeclamptic parturients in their study. In this study, a decreasing dose of 0.5% bupivacaine was practiced for the cesarean section. However, the incidence of hemodynamic change had not a significant difference (10mg versus 12.5mg). This finding was corresponding with a study done by Moshiri E et al. [40]. The result of our study showed that the mean gestational age in parturients with preeclampsia was considerably different compared with those of the non-preeclamptic parturients. This finding was in line with a study done by Sivevski A et al. [25].
Comparable to Sivevski A et al. finding [25], the result of our study showed that there was a statistically significant difference regarding the volume of preload taken between groups, which was higher in non-preeclamptic parturients compared to preeclamptic parturients (611.67 ml ± 289.65 VS 565.44 ml ± 318.45; p=0.004). In our study, intraoperative fluid consumption was lower in preeclamptic parturients compared with non-preeclamptic parturients (1463.41 ± 417.59 VS 1723.46 ml ± 352.41; p=0.001). This result was in line with Nikooseresht M et al. [10]. Similar to a study done by Lavie A et al. [36], in our study, the total estimated blood loss was comparable between groups, and no blood products were required throughout the procedure. Nikooseresht M et al. also found that the surgical durations were comparable between two groups [10]. This finding was in line with our study result.
In our study measurement of vasopressor consumption was difficult, due to the absence of standardized vasopressor usage in the hospital. Anesthetists were trying to manage hypotension with fluids and adrenaline accordingly. In this study, two parturients in the non-preeclamptic group were treated with adrenaline but there were no parturients treated with adrenaline in the preeclamptic groups. However, this difference was not statistically significant (p =0.550). Even though our study does not quantify it, studies found that hypotension requiring vasopressor medication(ephedrine and phenylephrine) following spinal anesthesia was less common in parturients with preeclampsia than in non-preeclamptic parturients [12,17,25,37,38,41,42]. The limitation of this study was the small sample size, observational study design which was difficult to control all possible co-founders(like oxytocin), and inability to quantify vasopressor consumption; due to lack of standardized vasopressor(ephedrine and phenylephrine)usage in the practice, which could affect the trends of hemodynamic change over time. As well the use of non-invasive blood pressure measurement in this study might miss some data which can be noticed in invasive blood pressure measurement Conclusion: This study showed that the incidence and magnitude of spinal anesthesia-induced hypotension in parturients undergone cesarean section were less in preeclamptics than in nonpreeclamptic parturients. In the preeclamptics group, they also experienced spinal anesthesiainduced hypotension, but the incidence and degree of hypotension were significantly lower than nonpreeclamptic parturients. Verbal and written consent was also secured before data collection.

Consent for publication
Not applicable.

Availability of data and materials
All data generated or analysed during this study are included in this published article

Funding
The entire funding to conduct this study was provided by University of Gondar College of Medicine and health science, Gondar, Ethiopia.

Competing interests
The authors declare that they have no competing interests.

Contributions
TY initiated the idea, carried out the study, and involved in drafting the manuscript. YW contributed to the statistical analysis and in preparing the manuscript. TY and HG contributed to the entry, analysis, critically revised the paper and provided the final version. MM edits starting from the proposal development up to the final manuscript writing and critically reviewed the manuscript for intellectual content. All authors read and approved the final manuscript.    Trends of diastolic blood pressure change following spinal anesthesia in non-preeclamptic and preeclamptic parturients, University of Gondar Northwest Ethiopia, May 2019(n=122).