Fig. 3

LMWF5A-induces changes in acetylated α-tubulin in HREC. a: An increase in total and perinuclear localization of acetylated α-tubulin is observed following LMWF5A treatment. Representative immunofluorescence staining for acetylated α-tubulin in HREC treated with Saline or LMWF5A for 3 or 6 hours (h). Acetylated α-tubulin in saline-treated controls is primarily located in microtubule organizing centers around the nucleus. LMWF5A treated HREC exhibit elevated cytoplasmic and perinuclear staining. Green represents conjugated Alexa fluor 488 anti-acetylated α-tubulin staining and blue represents nuclear 4′,6-diamidino-2-phenylindole DNA staining. b: Rapid increase in acetylated α-tubulin is observed following LMWF5A treatment. Western blot analysis of total protein extracts taken from HREC 10 min to 24 h post LMWF5A treatment as compared to total tubulin controls. Adapted and modified from Thomas et al 2016 [24]